CG Labz
Peptide Library / Longevity / Cellular

NAD+

Nicotinamide Adenine Dinucleotide

NAD+ Research Protocol

Complete Research Guide 4 min read1 peer-reviewed references

Quickstart Overview

NAD+ (nicotinamide adenine dinucleotide) is a central redox coenzyme and the substrate for sirtuins and PARPs — enzymes governing DNA repair, metabolism and aging. Injectable NAD+ is studied for cellular energy, longevity and recovery as levels decline with age.

Reconstitution
Supplied in solution or reconstitute per label
Literature Range
50–100 mg (SC); higher IV doses used clinically
Measuring Reference
Titrate from a low dose; slow administration
Frequency
Daily / several×/week
Route (studies)
SC (slow) or IV
Note
Inject slowly

Scientific Data

Molecular Formula
—
Molecular Weight
663.43 Da
Sequence
Dinucleotide coenzyme
Sequence Length
20
CAS
Research compound
Peptide Family
Coenzyme
Half-life
Refer to published literature
Solubility
Bacteriostatic water, sterile saline
Storage
Lyophilized: -20°C. Reconstituted: 2–8°C, use within 2–4 weeks.
Appearance
White lyophilized powder
Standard: 100 mg vial · 2 mL diluent

Dosing & Reconstitution Guide

Interactive Reconstitution
Adjust vial size, diluent and target dose. Results update live.
Full Precision Calculator →
Concentration
50.00 mg/mL
Draw Volume
2.000 mL
Syringe Units
200.0 U
Doses / Vial
1

Units assume a U-100 insulin syringe (100 units = 1 mL). Inject VERY slowly — rapid NAD+ causes flushing, nausea and chest tightness.

Frequency: Daily or several×/week

Reconstitution Steps

  1. 1Allow the lyophilized vial and bacteriostatic water to reach room temperature (~15 min).
  2. 2Swab both vial stoppers with a fresh 70% isopropyl alcohol pad and let them dry.
  3. 3Draw 2.0 mL of bacteriostatic water into a mixing syringe.
  4. 4Insert at an angle and let the water run slowly down the glass wall — never directly onto the powder.
  5. 5Gently swirl (do not shake) until the solution is completely clear.
  6. 6Label with date and concentration (mg/mL) and refrigerate at 2–8 °C immediately.

Supplies Needed

Planning example for a 8-week protocol at approximately 100000 mcg per day.

Peptide (100 mg vials)
Estimate vials from total mass: 100000 mcg/day × 56 days.
Bacteriostatic Water
One 10–30 mL vial typically covers a full cycle.
Insulin Syringes (U-100)
One per administration (29–31 G, 0.3–0.5 mL).
Alcohol Swabs
Two per administration (vial + site).
Sharps Container
Required for safe disposal.

Protocol Overview

Goal
Model NAD+ restoration for cellular energy and sirtuin activity.
Schedule
Daily/weekly, titrated.
Dose Range
50–100 mg SC per dose.

Dosing Protocol

Standard
50–100 mg slow subcutaneous, titrate up
Frequency
Daily or several times weekly
Target
100 mg/day as tolerated
Cycle Length
Ongoing or in blocks
Timing
Inject very slowly to avoid flushing
Route
Subcutaneous (slow)

Storage Instructions

Lyophilized
−20 °C long term (18–24 months); stable at room temperature for short transit.
Reconstituted
2–8 °C refrigerated; use within 4 weeks.
Light
Protect from direct light; keep in the original carton.
Freeze/Thaw
Avoid repeat freeze–thaw cycles after reconstitution.

Important Notes

  • Slow administration is essential — fast NAD+ is very uncomfortable (flushing, nausea).
  • Longevity evidence in humans is still early.

How This Works

NAD+ shuttles electrons in metabolism (glycolysis, the TCA cycle, oxidative phosphorylation) and is consumed by sirtuins (SIRT1-7) and PARPs during DNA repair and gene regulation. Cellular NAD+ falls with age and metabolic stress; restoring it is proposed to reactivate sirtuin-mediated maintenance pathways, improve mitochondrial function and support DNA repair. Injection bypasses the limited oral bioavailability of NAD+ itself.

Potential Benefits & Side Effects

Reported in research
  • Substrate for sirtuin and PARP maintenance pathways
  • Support for mitochondrial energy production
  • Studied for cellular energy, focus and recovery
  • Interest in DNA repair and longevity
Possible considerations
  • Flushing, nausea or chest tightness with fast injection (inject SLOWLY)
  • Injection-site discomfort
  • Transient cramping
  • Human longevity evidence is still preliminary

Lifestyle Factors

  • Adequate hydration and 7–9 h of sleep are standard controls in most research protocols.
  • Consistent dose timing improves the reliability of any self-tracked endpoints.
  • Maintain a clean, sterile workflow for every draw to avoid contamination.

Injection Technique

  1. 1Wash hands, then prep the vial top and the target site with separate alcohol swabs.
  2. 2Pinch a subcutaneous skin fold (abdomen, ~2 inches from the navel) and insert at 45–90°.
  3. 3Depress the plunger slowly over 3–5 seconds, hold 5 seconds, then withdraw.
  4. 4Rotate sites each administration and dispose of the syringe in a sharps container.

Frequently Asked Questions

Third-party HPLC testing

CG Labz Verified Batches

No published batches yet.

Research Disclaimer

All compounds are supplied strictly for laboratory research. Dosing figures below summarize ranges reported in published preclinical and clinical literature for reference purposes only. CG Labz does not provide medical advice or individualized dosing recommendations.

Peer-reviewed literature

References

  1. [1] Cell Metabolism
    Rajman L. et al. Therapeutic potential of NAD-boosting molecules.
    View Source