CG Labz
Peptide Library / Metabolic / Longevity

5-Amino-1MQ

NNMT Inhibitor

5-Amino-1MQ Research Protocol

Complete Research Guide 4 min read2 peer-reviewed references

Quickstart Overview

5-Amino-1MQ is a small-molecule inhibitor of nicotinamide N-methyltransferase (NNMT). By blocking NNMT it is studied for enhancing adipocyte energy metabolism and supporting NAD+ salvage, with interest in metabolic health and body composition.

Reconstitution
Add 2.0 mL bacteriostatic water to a 10 mg vial → 5 mg/mL (injectable form)
Literature Range
Oral 50–150 mg/day
Measuring Reference
At 5 mg/mL: 5 mg = 1.0 mL = 100 units
Frequency
Once daily
Class
NNMT inhibitor
Route (studies)
Oral or SC

Scientific Data

Molecular Formula
—
Molecular Weight
173.21 Da
Sequence
5-amino-1-methylquinolinium
Sequence Length
22
CAS
Research compound
Peptide Family
Small molecule
Half-life
Refer to published literature
Solubility
Bacteriostatic water, sterile saline
Storage
Lyophilized: -20°C. Reconstituted: 2–8°C, use within 2–4 weeks.
Appearance
White lyophilized powder
Standard: 10 mg vial · 2 mL diluent

Dosing & Reconstitution Guide

Interactive Reconstitution
Adjust vial size, diluent and target dose. Results update live.
Full Precision Calculator →
Concentration
5.00 mg/mL
Draw Volume
1.000 mL
Syringe Units
100.0 U
Doses / Vial
2

Units assume a U-100 insulin syringe (100 units = 1 mL). Available orally and as an injectable; NNMT inhibition is the key mechanism.

Frequency: Once daily

Reconstitution Steps

  1. 1Allow the lyophilized vial and bacteriostatic water to reach room temperature (~15 min).
  2. 2Swab both vial stoppers with a fresh 70% isopropyl alcohol pad and let them dry.
  3. 3Draw 2.0 mL of bacteriostatic water into a mixing syringe.
  4. 4Insert at an angle and let the water run slowly down the glass wall — never directly onto the powder.
  5. 5Gently swirl (do not shake) until the solution is completely clear.
  6. 6Label with date and concentration (mg/mL) and refrigerate at 2–8 °C immediately.

Supplies Needed

Planning example for a 12-week protocol at approximately 5000 mcg per day.

Peptide (10 mg vials)
Estimate vials from total mass: 5000 mcg/day × 84 days.
Bacteriostatic Water
One 10–30 mL vial typically covers a full cycle.
Insulin Syringes (U-100)
One per administration (29–31 G, 0.3–0.5 mL).
Alcohol Swabs
Two per administration (vial + site).
Sharps Container
Required for safe disposal.

Protocol Overview

Goal
Model NNMT inhibition on adipocyte metabolism and NAD+.
Schedule
Daily, 8–12 weeks.
Dose Range
Oral 50–150 mg/day.

Dosing Protocol

Oral
50–150 mg daily reported in protocols
Injectable
Lower equivalent doses used
Frequency
Once daily
Cycle Length
8–12 weeks
Timing
Morning
Route
Oral or subcutaneous

Storage Instructions

Lyophilized
−20 °C long term (18–24 months); stable at room temperature for short transit.
Reconstituted
2–8 °C refrigerated; use within 4 weeks.
Light
Protect from direct light; keep in the original carton.
Freeze/Thaw
Avoid repeat freeze–thaw cycles after reconstitution.

Important Notes

  • A small molecule, not a peptide; stable and usable orally.
  • Human efficacy evidence is still preliminary.

How This Works

NNMT is highly expressed in white adipose tissue and consumes nicotinamide (an NAD+ precursor) and methyl groups. Inhibiting NNMT with 5-Amino-1MQ raises cellular NAD+ and SAM levels, increases the NAD+/NADH ratio and energy expenditure in fat cells, and in preclinical models reduces adiposity while preserving lean mass. It is taken orally or by injection in research settings.

Potential Benefits & Side Effects

Reported in research
  • Increased adipocyte energy expenditure in models
  • Support for NAD+ salvage via NNMT inhibition
  • Reduced fat mass with preserved lean mass in animal studies
  • Interest in metabolic and longevity research
Possible considerations
  • Limited human data
  • Mild GI effects reported with oral use
  • Injection-site reactions (injectable form)
  • Long-term safety not established

Lifestyle Factors

  • Adequate hydration and 7–9 h of sleep are standard controls in most research protocols.
  • Consistent dose timing improves the reliability of any self-tracked endpoints.
  • Maintain a clean, sterile workflow for every draw to avoid contamination.

Injection Technique

  1. 1Wash hands, then prep the vial top and the target site with separate alcohol swabs.
  2. 2Pinch a subcutaneous skin fold (abdomen, ~2 inches from the navel) and insert at 45–90°.
  3. 3Depress the plunger slowly over 3–5 seconds, hold 5 seconds, then withdraw.
  4. 4Rotate sites each administration and dispose of the syringe in a sharps container.

Frequently Asked Questions

Third-party HPLC testing

CG Labz Verified Batches

No published batches yet.

Research Disclaimer

All compounds are supplied strictly for laboratory research. Dosing figures below summarize ranges reported in published preclinical and clinical literature for reference purposes only. CG Labz does not provide medical advice or individualized dosing recommendations.

Peer-reviewed literature

References

  1. [1] Nature
    Kraus D. et al. Nicotinamide N-methyltransferase knockdown protects against diet-induced obesity.
    View Source
  2. [2] Biochemistry
    Neelakantan H. et al. Selective and membrane-permeable small-molecule inhibitors of NNMT.
    View Source