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Peptide Library / Immune / Longevity

Thymosin Alpha-1

Thymalfasin

Thymosin Alpha-1 (Thymalfasin) Research Protocol

Complete Research Guide 4 min read1 peer-reviewed references

Quickstart Overview

Thymosin Alpha-1 is a 28-amino-acid thymic peptide (marketed as thymalfasin) approved in many countries as an immune modulator. It is studied for enhancing T-cell responses in infection, immune senescence and as an adjuvant in vaccination and cancer immunotherapy.

Reconstitution
Add 2.0 mL bacteriostatic water to a 5 mg vial → 2.5 mg/mL
Clinical Dose
1.6 mg twice weekly
Measuring Reference
At 2.5 mg/mL: 1.6 mg = 0.64 mL = 64 units
Frequency
2× weekly
Route (studies)
Subcutaneous
Also known as
Thymalfasin, Zadaxin

Scientific Data

Molecular Formula
—
Molecular Weight
3108.3 Da
Sequence
Ac-SDAAVDTSSEITTKDLKEKKEVVEEAEN
Sequence Length
30
CAS
Research compound
Peptide Family
Thymic peptide
Half-life
Refer to published literature
Solubility
Bacteriostatic water, sterile saline
Storage
Lyophilized: -20°C. Reconstituted: 2–8°C, use within 2–4 weeks.
Appearance
White lyophilized powder
Standard: 5 mg vial · 2 mL diluent

Dosing & Reconstitution Guide

Interactive Reconstitution
Adjust vial size, diluent and target dose. Results update live.
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Concentration
2.50 mg/mL
Draw Volume
0.640 mL
Syringe Units
64.0 U
Doses / Vial
3

Units assume a U-100 insulin syringe (100 units = 1 mL). The clinical dose is 1.6 mg twice weekly; very well tolerated.

Frequency: 2× weekly

Reconstitution Steps

  1. 1Allow the lyophilized vial and bacteriostatic water to reach room temperature (~15 min).
  2. 2Swab both vial stoppers with a fresh 70% isopropyl alcohol pad and let them dry.
  3. 3Draw 2.0 mL of bacteriostatic water into a mixing syringe.
  4. 4Insert at an angle and let the water run slowly down the glass wall — never directly onto the powder.
  5. 5Gently swirl (do not shake) until the solution is completely clear.
  6. 6Label with date and concentration (mg/mL) and refrigerate at 2–8 °C immediately.

Supplies Needed

Planning example for a 8-week protocol at approximately 1600 mcg per day.

Peptide (5 mg vials)
Estimate vials from total mass: 1600 mcg/day × 56 days.
Bacteriostatic Water
One 10–30 mL vial typically covers a full cycle.
Insulin Syringes (U-100)
One per administration (29–31 G, 0.3–0.5 mL).
Alcohol Swabs
Two per administration (vial + site).
Sharps Container
Required for safe disposal.

Protocol Overview

Goal
Model thymic T-cell immune modulation.
Schedule
2× weekly, weeks–months.
Dose Range
1.6 mg twice weekly.

Dosing Protocol

Standard
1.6 mg twice weekly (clinical dose)
Frequency
2× weekly
Acute support
Higher-frequency short courses in some protocols
Cycle Length
Weeks to months
Timing
Not time-critical
Route
Subcutaneous

Storage Instructions

Lyophilized
−20 °C long term (18–24 months); stable at room temperature for short transit.
Reconstituted
2–8 °C refrigerated; use within 4 weeks.
Light
Protect from direct light; keep in the original carton.
Freeze/Thaw
Avoid repeat freeze–thaw cycles after reconstitution.

Important Notes

  • Well tolerated; use caution in autoimmune contexts where immune stimulation is undesired.
  • Among the most clinically validated peptides in the catalog.

How This Works

Thymosin Alpha-1 modulates both innate and adaptive immunity, primarily by promoting maturation and differentiation of T-cells via Toll-like-receptor signalling (notably TLR9/TLR2) on dendritic cells. It increases Th1 cytokines, enhances antigen presentation and natural-killer activity, and helps restore immune balance in states of immunosuppression or chronic infection. It is used clinically for hepatitis B/C and as an immune adjuvant.

Potential Benefits & Side Effects

Reported in research
  • Enhanced T-cell maturation and Th1 immune response
  • Improved antigen presentation and NK-cell activity
  • Clinical use in chronic hepatitis and as a vaccine adjuvant
  • Restoration of immune balance in immunosuppression
Possible considerations
  • Injection-site reactions (common, mild)
  • Transient fatigue
  • Generally very well tolerated
  • Theoretical caution in autoimmune conditions

Lifestyle Factors

  • Adequate hydration and 7–9 h of sleep are standard controls in most research protocols.
  • Consistent dose timing improves the reliability of any self-tracked endpoints.
  • Maintain a clean, sterile workflow for every draw to avoid contamination.

Injection Technique

  1. 1Wash hands, then prep the vial top and the target site with separate alcohol swabs.
  2. 2Pinch a subcutaneous skin fold (abdomen, ~2 inches from the navel) and insert at 45–90°.
  3. 3Depress the plunger slowly over 3–5 seconds, hold 5 seconds, then withdraw.
  4. 4Rotate sites each administration and dispose of the syringe in a sharps container.

Frequently Asked Questions

Third-party HPLC testing

CG Labz Verified Batches

No published batches yet.

Research Disclaimer

All compounds are supplied strictly for laboratory research. Dosing figures below summarize ranges reported in published preclinical and clinical literature for reference purposes only. CG Labz does not provide medical advice or individualized dosing recommendations.

Peer-reviewed literature

References

  1. [1] Annals of the New York Academy of Sciences
    Garaci E. et al. Thymosin alpha 1: an immune modulator.
    View Source