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Peptide Library / Cognition / Neuro

DSIP

Delta Sleep-Inducing Peptide

DSIP (Delta Sleep-Inducing Peptide) Research Protocol

Complete Research Guide 4 min read1 peer-reviewed references

Quickstart Overview

DSIP is a naturally occurring nonapeptide first isolated from the blood of sleeping rabbits. It is studied for promoting slow-wave (delta) sleep, modulating stress hormones and stabilising circadian and neuro-endocrine rhythms.

Reconstitution
Add 2.0 mL bacteriostatic water to a 5 mg vial → 2.5 mg/mL
Literature Range
100–300 mcg per administration
Measuring Reference
At 2.5 mg/mL: 200 mcg = 0.08 mL = 8 units
Frequency
Nightly
Route (studies)
Subcutaneous
Cycle
2–4 weeks

Scientific Data

Molecular Formula
—
Molecular Weight
848.9 Da
Sequence
Trp-Ala-Gly-Gly-Asp-Ala-Ser-Gly-Glu
Sequence Length
27
CAS
Research compound
Peptide Family
Neuropeptide
Half-life
Refer to published literature
Solubility
Bacteriostatic water, sterile saline
Storage
Lyophilized: -20°C. Reconstituted: 2–8°C, use within 2–4 weeks.
Appearance
White lyophilized powder
Standard: 5 mg vial · 2 mL diluent

Dosing & Reconstitution Guide

Interactive Reconstitution
Adjust vial size, diluent and target dose. Results update live.
Full Precision Calculator →
Concentration
2.50 mg/mL
Draw Volume
0.080 mL
Syringe Units
8.0 U
Doses / Vial
25

Units assume a U-100 insulin syringe (100 units = 1 mL). Dose about an hour before bed; response varies between individuals.

Frequency: Nightly

Reconstitution Steps

  1. 1Allow the lyophilized vial and bacteriostatic water to reach room temperature (~15 min).
  2. 2Swab both vial stoppers with a fresh 70% isopropyl alcohol pad and let them dry.
  3. 3Draw 2.0 mL of bacteriostatic water into a mixing syringe.
  4. 4Insert at an angle and let the water run slowly down the glass wall — never directly onto the powder.
  5. 5Gently swirl (do not shake) until the solution is completely clear.
  6. 6Label with date and concentration (mg/mL) and refrigerate at 2–8 °C immediately.

Supplies Needed

Planning example for a 4-week protocol at approximately 200 mcg per day.

Peptide (5 mg vials)
Estimate vials from total mass: 200 mcg/day × 28 days.
Bacteriostatic Water
One 10–30 mL vial typically covers a full cycle.
Insulin Syringes (U-100)
One per administration (29–31 G, 0.3–0.5 mL).
Alcohol Swabs
Two per administration (vial + site).
Sharps Container
Required for safe disposal.

Protocol Overview

Goal
Model delta-sleep promotion and HPA modulation.
Schedule
Nightly, 2–4 weeks.
Dose Range
100–300 mcg/dose.

Dosing Protocol

Standard
100–300 mcg before sleep
Frequency
Nightly or as needed
Target
200 mcg typical
Cycle Length
2–4 weeks
Timing
~1 hour before bed
Route
Subcutaneous

Storage Instructions

Lyophilized
−20 °C long term (18–24 months); stable at room temperature for short transit.
Reconstituted
2–8 °C refrigerated; use within 4 weeks.
Light
Protect from direct light; keep in the original carton.
Freeze/Thaw
Avoid repeat freeze–thaw cycles after reconstitution.

Important Notes

  • Individual response varies considerably.
  • Also studied for stress-hormone modulation beyond sleep.

How This Works

DSIP crosses the blood-brain barrier and interacts with multiple neurotransmitter and neuro-endocrine systems. It promotes delta-wave (deep) sleep, modulates the hypothalamic-pituitary-adrenal axis (lowering stress-related cortisol), influences LH and GH secretion, and shows antioxidant and stress-protective activity. Its precise receptor remains uncharacterised, but its circadian and anti-stress effects are consistently reported.

Potential Benefits & Side Effects

Reported in research
  • Promotion of slow-wave (deep) sleep
  • Lowered stress-related cortisol (HPA modulation)
  • Circadian-rhythm stabilisation
  • Antioxidant and stress-protective effects reported
Possible considerations
  • Injection-site reactions
  • Variable individual response
  • Possible mild grogginess
  • Limited large-scale human data

Lifestyle Factors

  • Adequate hydration and 7–9 h of sleep are standard controls in most research protocols.
  • Consistent dose timing improves the reliability of any self-tracked endpoints.
  • Maintain a clean, sterile workflow for every draw to avoid contamination.

Injection Technique

  1. 1Wash hands, then prep the vial top and the target site with separate alcohol swabs.
  2. 2Pinch a subcutaneous skin fold (abdomen, ~2 inches from the navel) and insert at 45–90°.
  3. 3Depress the plunger slowly over 3–5 seconds, hold 5 seconds, then withdraw.
  4. 4Rotate sites each administration and dispose of the syringe in a sharps container.

Frequently Asked Questions

Third-party HPLC testing

CG Labz Verified Batches

No published batches yet.

Research Disclaimer

All compounds are supplied strictly for laboratory research. Dosing figures below summarize ranges reported in published preclinical and clinical literature for reference purposes only. CG Labz does not provide medical advice or individualized dosing recommendations.

Peer-reviewed literature

References

  1. [1] Experientia
    Schoenenberger G.A. & Monnier M. Characterization of delta-sleep-inducing peptide.
    View Source