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Peptide Library / Sexual Health / Hormone

Kisspeptin-10

KISS1 (112-121) / Metastin fragment

Kisspeptin-10 Research Protocol

Complete Research Guide 4 min read1 peer-reviewed references

Quickstart Overview

Kisspeptin-10 is the 10-amino-acid active fragment of kisspeptin, the master upstream regulator of the reproductive axis. It stimulates GnRH release, driving endogenous LH, FSH and downstream sex-hormone production, and is studied in fertility and hormonal research.

Reconstitution
Add 2.0 mL bacteriostatic water to a 5 mg vial → 2.5 mg/mL
Literature Range
mcg-range, study-dependent
Measuring Reference
At 2.5 mg/mL: 250 mcg = 0.1 mL = 10 units
Frequency
Daily/pulsatile
Route (studies)
Subcutaneous
Target
KISS1R/GPR54

Scientific Data

Molecular Formula
—
Molecular Weight
1302.5 Da
Sequence
YNWNSFGLRF-NH2
Sequence Length
12
CAS
Research compound
Peptide Family
Neuropeptide
Half-life
Refer to published literature
Solubility
Bacteriostatic water, sterile saline
Storage
Lyophilized: -20°C. Reconstituted: 2–8°C, use within 2–4 weeks.
Appearance
White lyophilized powder
Standard: 5 mg vial · 2 mL diluent

Dosing & Reconstitution Guide

Interactive Reconstitution
Adjust vial size, diluent and target dose. Results update live.
Full Precision Calculator →
Concentration
2.50 mg/mL
Draw Volume
0.100 mL
Syringe Units
10.0 U
Doses / Vial
20

Units assume a U-100 insulin syringe (100 units = 1 mL). Acts upstream of GnRH; short half-life favours pulsatile dosing.

Frequency: Daily/pulsatile

Reconstitution Steps

  1. 1Allow the lyophilized vial and bacteriostatic water to reach room temperature (~15 min).
  2. 2Swab both vial stoppers with a fresh 70% isopropyl alcohol pad and let them dry.
  3. 3Draw 2.0 mL of bacteriostatic water into a mixing syringe.
  4. 4Insert at an angle and let the water run slowly down the glass wall — never directly onto the powder.
  5. 5Gently swirl (do not shake) until the solution is completely clear.
  6. 6Label with date and concentration (mg/mL) and refrigerate at 2–8 °C immediately.

Supplies Needed

Planning example for a 6-week protocol at approximately 250 mcg per day.

Peptide (5 mg vials)
Estimate vials from total mass: 250 mcg/day × 42 days.
Bacteriostatic Water
One 10–30 mL vial typically covers a full cycle.
Insulin Syringes (U-100)
One per administration (29–31 G, 0.3–0.5 mL).
Alcohol Swabs
Two per administration (vial + site).
Sharps Container
Required for safe disposal.

Protocol Overview

Goal
Model upstream HPG-axis (GnRH) stimulation.
Schedule
Daily/pulsatile, weeks.
Dose Range
mcg-range per protocol.

Dosing Protocol

Standard
Study-dependent; mcg-range, pulsatile
Frequency
Daily or per protocol
Target
Per research design
Cycle Length
Weeks
Timing
Short half-life favours pulsatile dosing
Route
Subcutaneous

Storage Instructions

Lyophilized
−20 °C long term (18–24 months); stable at room temperature for short transit.
Reconstituted
2–8 °C refrigerated; use within 4 weeks.
Light
Protect from direct light; keep in the original carton.
Freeze/Thaw
Avoid repeat freeze–thaw cycles after reconstitution.

Important Notes

  • Short half-life means frequent/pulsatile dosing.
  • Continuous high dosing can desensitise the receptor.

How This Works

Kisspeptin neurons sit at the top of the reproductive (HPG) axis. Kisspeptin-10 binds the KISS1R (GPR54) receptor on GnRH neurons, triggering pulsatile GnRH release, which in turn drives pituitary LH and FSH and therefore testicular/ovarian sex-hormone output. Because it acts upstream and physiologically, it produces a natural hormonal cascade and is studied as a more physiological alternative to direct gonadotropin stimulation.

Potential Benefits & Side Effects

Reported in research
  • Stimulated endogenous GnRH → LH/FSH → sex hormones
  • Physiological, upstream HPG-axis activation
  • Studied for fertility and hypogonadism
  • Short-acting, pulsatile-friendly profile
Possible considerations
  • Injection-site reactions
  • Short half-life (frequent dosing needed)
  • Desensitisation risk with continuous high dosing
  • Limited long-term data

Lifestyle Factors

  • Adequate hydration and 7–9 h of sleep are standard controls in most research protocols.
  • Consistent dose timing improves the reliability of any self-tracked endpoints.
  • Maintain a clean, sterile workflow for every draw to avoid contamination.

Injection Technique

  1. 1Wash hands, then prep the vial top and the target site with separate alcohol swabs.
  2. 2Pinch a subcutaneous skin fold (abdomen, ~2 inches from the navel) and insert at 45–90°.
  3. 3Depress the plunger slowly over 3–5 seconds, hold 5 seconds, then withdraw.
  4. 4Rotate sites each administration and dispose of the syringe in a sharps container.

Frequently Asked Questions

Third-party HPLC testing

CG Labz Verified Batches

No published batches yet.

Research Disclaimer

All compounds are supplied strictly for laboratory research. Dosing figures below summarize ranges reported in published preclinical and clinical literature for reference purposes only. CG Labz does not provide medical advice or individualized dosing recommendations.

Peer-reviewed literature

References

  1. [1] Journal of Clinical Investigation
    Dhillo W.S. et al. Kisspeptin-54 stimulates the HPG axis in humans.
    View Source